3 People, Including 1-Year-Old Child, Killed in Wrong-Way I-65 Crash in Indiana

LOUISVILLE, Ky. (Aug. 23, 2026) — Three people, including a 1-year-old child, were killed early Sunday morning in a wrong-way crash on Interstate 65 near Seymour, Indiana, according to Indiana State Police.

Police said a caller reported a head-on collision on the southbound lanes of I-65, just south of the 48-mile marker and about one mile south of Seymour. Responding troopers found multiple people had died and requested assistance from the Indiana State Police Crash Reconstruction Team.

Investigators believe a 2005 Jeep Liberty driven by 51-year-old Theosco Middlebrooks of Louisville was traveling north in the southbound lanes when it collided head-on with a 2023 Ford Escape.

The Ford was driven by 31-year-old Alyssa Osborn of Scottsburg. Her 1-year-old son was also inside the vehicle.

Middlebrooks and Osborn died at the scene, police said. Osborn’s son was transported to Norton Children’s Hospital in Louisville, where he later died.

Investigators said alcohol consumption by Middlebrooks may have contributed to the crash. Toxicology results remain pending, and authorities have not made a final determination about the cause.

The crash forced authorities to close the southbound lanes of I-65 for approximately four hours while emergency crews and investigators worked at the scene.

Separately, Louisville Metro Police are investigating a rollover crash near downtown Louisville. Officers responded to the intersection of Roy Wilkins Avenue and West Chestnut Street shortly before 7:10 p.m. Sunday after a passenger vehicle and an SUV were involved in a collision.

What to Know About This Wrong-Way Crash in Louisville and Southern Indiana

Fatal crashes may involve investigations into vehicle movement, roadway conditions, impairment, evidence, and other contributing factors.

1. Wrong-Way Crash Investigations

Investigators may review traffic signs, roadway markings, vehicle positions, and other evidence to determine how the wrong-way crash occurred.

2. Crash Reconstruction

Vehicle damage, debris, tire marks, roadway conditions, and witness statements may help investigators reconstruct the collision.

3. Suspected Alcohol or Impairment

Toxicology testing may be conducted when impairment is suspected. Pending results do not confirm impairment.

4. Child Passenger Safety

Investigators may review seating positions and whether child passengers were properly secured.

5. Fatal Crash Investigation

Authorities may continue examining evidence before determining the crash’s cause or contributing factors.

6. Roadway Closures and Emergency Response

Serious crashes may require temporary closures for emergency response and investigation. Southbound I-65 was closed for approximately four hours.

Notes

This report is based on preliminary information from Indiana State Police and Louisville Metro Police regarding an I-65 collision near Seymour, Indiana. Three people, including a 1-year-old child, died after a 2005 Jeep Liberty traveling the wrong way collided head-on with a 2023 Ford Escape. Alcohol may have contributed, but toxicology results remain pending, and authorities have not made a final determination regarding the cause.

Disclaimer

This article is a summary of publicly available information and is intended for general informational purposes only. It does not constitute legal advice and does not include unverified personal details.